Electrospinning technique has excellent advantages such as tunable functionality, thin fibers with large surface areas, ease of processing and good physical properties. Electrospinning provides wide usage area with these advantages in biomedical applications. Polylactic acid (PLA) is a biodegradable and biocompatible polymer, so it can be used in various biomedical applications. PLA can be easily electrospun from solution by using different kinds of conventional solvents. Electrospun PLA based nanofibers are used in many biomedical applications such as drug delivery, scaffold for tissue engineering, dressings for wound healing, dental applications etc. This review focuses on electrospun PLA based nanofibers used in biomedical applications in recent years. Future perspectives of electrospun PLA based fibers are also discussed in the last part.