The development of biomedical electronics, biosynthesis of ZnS nanoparticles(NPs) much attracted researchers, due to an eco-friendly and cost-effective routes forsynthesisZnSnanoparticles. In this present work, ZnS NPswas synthesized by using acalypha indica and curcuma longa plant extract using chemical co-precipitation method. The structural, morphological, element composition of biosynthesisZnS NPs was characterized by XRD, SEM and EDAX respectively. Optical and photoluminescence (PL) properties were evaluated by UV Visible spectroscopy. The formation of inhibition zone diameter against human pathogenic microorganisms was screened by in vitro disc diffusion method. From this investigation formation of inhibition zones clearly shows biosynthesizedZnS NPs have high antimicrobial activity against tested organisms, especially curcuma longa plant extract mediated ZnS NPs was formed maximum inhibition against all the tested microorganism.