A simple, specific and precise high performance liquid chromatographic method has been developed for estimation of Glipizide in its pure and pharmaceutical dosage forms. A HPLC system consisting of a gradient pump, reverse phase C-18 analytical column, a variable UV-Visible detector set at 225 nm and an integrator was used. The mobile phase consisted of methanol and 0.115%w/v ammonium dihydrogen phosphate buffer and pumped at 1ml/min at ambient temperature. This RP-HPLC method was validated for its linearity, precision and accuracy. The calibration curve was linear in the range of 10 to 70 μg/ml).