An experimental investigation has been carried out to understand the effect of split injection concepts on the emission level particularly on the reduction of Nitrogen oxide emission is accompanied with the reduction of Brake thermal efficiency, hence to offset the reduction of thermal efficiency blend of diesel and oxygenated compound Di-ethylene glycol have been used as fuel for the analysis.Thus the emission characteristics of diesel- Di-ethylene glycol are investigated using the single cylinder direct injection naturally aspirated diesel engine using split injection method involving double lobed cams. The double lobed cam was designed to inject fuel in the proportion of 40-60% with an interval of 8Ú between pilot and main injection. The oxygenated compounds Di-ethylene glycol is blended with diesel fuel in the proportion 5% and 7% by volume. The engine was tested using Eddy current dynamometer at a speed of 1500 r.p.m from no load to full load using diesel, 5% and 7% diesel-Diethylene Glycol blend using single injection and split injection. The AVL smoke meter and krypton gas analyzer are used for measuring emission parameters values. The emission curves such as CO, CO2, HC, NOx and smoke with respect to brake power are plotted for both single injection and single injection and compared. From the results, it is found that NOx emission is drastically reduced with split injection using diesel but slightly higher with diesel- Di-ethylene Glycol blend.